Place this clip at the reaction beat after the mistake is shown, then follow it with the emotional damage meme sound for a second hit, or swap that follow-up for the sad meow song when the edit needs a longer comedic cooldown.
Über diesen Sound
This five-second clip is Kazuma Kiryu singing the 'Dame Da Ne' line from Baka Mitai, delivered as a soft, earnest karaoke vocal. It feels sincere and heartbroken, which makes even a small disappointment land as melodramatic and funny. It comes from the karaoke minigame in Yakuza 0.