Place it at the exact frame the loss becomes obvious, as the closing beat of the edit. Lead in with It Was At This Moment He Knew for the setup, then let this take the payoff, or swap to the Darth Vader No Sound version when you want the scored take instead.
Über diesen Sound
This is Darth Vader's extended no, presented as a clean vocal take with no score underneath, running about seven seconds. The strained vocoder delivery climbs into desperate anguish, making it feel both heartbroken and absurdly overblown at once. It comes from Star Wars: Episode III – Revenge of the Sith, where Vader reacts to learning Padmé has died.