The line comes from the 1980 comedy Airplane!, written and directed by Jim Abrahams, David Zucker and Jerry Zucker. Leslie Nielsen plays Dr Rumack, a straight-faced doctor on a flight in crisis, and the Shirley joke is one of the film's most quoted exchanges. It spread through television repeats, home video and later meme culture, where the deadpan delivery made it perfect for reaction clips and short edits. Decades later it still works because the pun is simple, the setup is familiar, and the humour comes from saying something absurd with total seriousness.
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The classic 'Don't call me Shirley' line from Airplane, delivered deadpan after 'Surely you can't be serious.' Use it to correct someone with dry humour.
This is the deadpan punchline from Airplane!, when Dr Rumack replies to a passenger who says, 'Surely you can't be serious,' with the flat correction, 'I am serious, and don't call me Shirley.' Leslie Nielsen delivers it in a calm, completely straight voice, with no pause for laughter and no hint that he is making a joke, which is exactly why the wordplay lands so hard. The clip is short, dry and instantly recognisable, turning a simple name correction into a sarcastic shutdown. It works because the delivery sounds serious while the joke is pure silliness, making listeners smile at the absurd contrast.