Put it at the end of a short setup where someone is about to take something, after No No Wait Wait builds the warning and before Eww Brother Eww adds a second reaction. Keep it as the single reaction in the scene so the grab stays the focus.
Über diesen Sound
This six-second spoken reaction clip delivers a sharp, indignant 'don't touch my butt' in a firm, annoyed voice. It feels cheeky and confrontational, turning an awkward overstep into a joke that lands because the refusal is so direct. The delivery makes it sound like a personal boundary being set in the middle of a playful argument.