Place it at the end of a build-up where a character keeps making the same mistake, after Dun Dun Dun signals the trouble ahead and before Vine Boom lands the reaction, so the sequence moves from warning to payoff.
Über diesen Sound
This 3.6-second clip is a long, rattling growl that breaks into a sharp shouted 'That's it!' from a cartoon voice pushed past patience. It feels fed-up, comic and suddenly confrontational, like the exact second a joke stops being funny. It comes from episode 14 of Battle for Dream Island Again, known as BFDIA.