Place it at the exact beat where a challenge is issued, after the setup line and before your response shot. Build extra comic contrast by following it with I'm Batman for a second mock-heroic claim, or lead in with a Metal Gear Alert to raise the stakes before Homer answers.
Über diesen Sound
This is a short 2.3-second voice clip of Homer delivering a Clint Eastwood style moment, built around his familiar cartoon delivery pushed into a tougher, deadpan register. It feels funny and slightly tense at once, because the bravado does not match the character you expect to hear it from, which makes the impression land as a quick, playful standoff.