Place it immediately after the losing hand is shown, then follow with the Windows XP Error sound for a system-fail beat before cutting to the winner's reaction. For a slower defeat, pair it after the SpongeBob Sad Song instead, letting that cue set up the complaint.
Über diesen Sound
This is a short frustrated voice clip built around losing at rock paper scissors, lasting just under fourteen seconds. The speaker sounds fed up and defeated, turning a simple hand game into a full vent about bad luck and unfair rounds. It feels funny because the anger is far bigger than the game deserves, making it easy to recognise after any sudden loss.