Place it right after the moment of rejection in your edit, then follow it with the sad violin for a slow finish that makes the snub land much bigger for viewers.
Über diesen Sound
This short clip is a single spoken line, lasting about two and a half seconds, delivered in a hurt, pleading voice that sounds like someone has just been pushed away. It feels sad and a little awkward, turning a small rejection into a dramatic moment. The line comes from Smosh, credited in 2014.