The line comes from Filthy Frank, the chaotic YouTube character created by George Miller, who later became known as Joji. Frank's exasperated delivery turned it's time to stop into a widely reused reaction clip for calling out behaviour that had gone too far. It spread through edits, compilations and soundboards in the mid 2010s, and quieter versions like this one appeared so creators could layer it under other audio without clipping. It remains popular because the message is instant and needs no context to understand.