Lain is the quiet protagonist of Serial Experiments Lain, the 1998 anime series known for its exploration of identity, technology and the boundary between the real world and the Wired. Her laugh appears in the series opening sequence, a moment fans singled out because it contrasts so sharply with her usually reserved manner. Clips of the intro circulated among anime fans and meme editors, who reused the laugh as shorthand for uncanny, off-kilter humour. It remains popular because it is instantly recognisable to fans and works without any context.