The falling whistle belongs to the classic Warner Bros cartoon sound library used throughout Looney Tunes and Merrie Melodies shorts from the golden age of animation. It became inseparable from Wile E. Coyote and the Road Runner, created by Chuck Jones in 1949, whose repeated cliff falls made the slide whistle descent a shorthand for cartoon gravity. As those shorts reran on television for decades and clips later circulated online, the effect survived as a universal meme sound for drops, failures and long falls.
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The classic cartoon falling whistle from Looney Tunes, heard when Wile E. Coyote drops off a cliff in Road Runner cartoons and used for fail moments.
This is the long descending whistle that plays as a character falls from a great height, starting high and sliding steadily down in pitch until it fades or ends with the impact. It is most associated with Wile E. Coyote plummeting off a desert cliff while chasing the Road Runner, the delay building the joke before gravity wins. The sound instantly signals a doomed drop, a plan gone wrong, or something sinking fast, and viewers understand the gag even before anything hits the ground.