Put it at the end of a short setup, right after the single letter appears, then follow it with an emotional damage reaction for the fallout and a bruh as the final reply.
Über diesen Sound
The clip is a single vocal A stretched into a very long shout, held until it turns into an exaggerated scream. It feels absurd and over the top, like a small sound that refuses to stop. The drawn-out delivery makes it funny rather than alarming, for a moment that needs to be blown out of proportion.