Put it at the end of a fail sequence once the mistake is fully visible, then follow it with the damn meme sound button for a second reaction, or place a wrong answer gameshow buzz just before it to set up the error cleanly.
Über diesen Sound
A short voice clip, under two seconds, of someone shouting the Spanish word 'Maldito' in a sharp, annoyed burst. It lands as a dramatic little curse, the kind of exasperated reaction that turns a small annoyance into a funny, over-the-top complaint without needing any extra explanation or setup from the listener at all.