Place it immediately after the rude line in your edit, then follow it with Dun Dun Dun to hold the pause, or cut to SpongeBob Fail when the comeback falls flat. Keep it as the single reaction in the scene so the next sound does the payoff.
Über diesen Sound
This is a very short, under-two-second clip of Mr Rude's voice processed with the G-Major effect, so the familiar cartoon voice comes out low, warped and slightly off-key. It feels jarring and deliberately rude, funny because the distortion makes a small remark sound exaggerated and confrontational, and it lands best as a sudden interruption rather than a gentle reaction.