Place it right after the failed moment in your edit, then follow it with the Sad Violin for a dramatic comedic fall, or put the Eww Brother Eww before it to set up the reaction first.
Über diesen Sound
This is an eight-second vocal groan, a single stretched-out 'ughhh' delivered in a weary, exasperated tone with no music or other effects. It lands as pure frustration and disgust, the sound of someone giving up on explaining something annoying. The drawn-out delivery makes the irritation feel bigger and funnier, turning a small complaint into an over-the-top reaction.