Place it at the end of a setup where a player presents a hopeless plan, then follow it with Nope for a second refusal that closes the scene. For a bigger reaction, put No God Please No before it so the denial reads as the final verdict.
Über diesen Sound
This is a very short spoken no from Age of Empires II, lasting under a second and delivered in a flat game voice with no extra music or effects. It feels blunt, dry and instantly dismissive, the kind of refusal that lands funnier because it offers no explanation. The line comes from Age of Empires II, where units give short replies to player commands.