Place it at the climax of a fail edit, just after the mistake becomes clear, then follow it with the Shocked Meme Sound and the Goofy Yell to keep the reaction going.
Über diesen Sound
This ten-second clip is a single panicked voice repeating 'noo, noo, noo' before it breaks into one long, drawn-out scream. The refusal starts shaky and gets louder and more desperate with each repeat, until the scream takes over completely. It feels frantic and over-the-top, like someone watching a disaster unfold and knowing they cannot stop it.