Put it near the end of a sequence showing thankless effort, after the work is visible and before the reaction lands. Follow it with the It Was At This Moment He Knew sound when the effort turns out to have been wasted, or use the Fail Sound Meme sound when the thank-you never arrives.
Über diesen Sound
This is a short spoken line, just under four seconds long, in which a man called Norman asks how much he has sacrificed. The question is framed as a complaint, putting the focus on everything he gave up and what it cost him. It feels bitter, resentful and self-pitying, like someone demanding recognition for losses no one else seems to count.