Put it at the end of a failed guess, after the reveal, then follow it with Vine Boom for a bigger reaction or SpongeBob Fail to keep the joke going; use Dun Dun Dun before the answer for extra payoff.
Über diesen Sound
This is a short, low-pitched buzzer lasting just over a second, with a deep, flat tone that cuts straight through a quiet room. It lands with instant, awkward disappointment, the kind of sound that makes a wrong guess feel final and a little funny at the same time.