Place it as the immediate reaction after a plan goes wrong, then follow with the Darth Vader Nooo for a bigger second meltdown, or precede it with the Fail Sound Meme to set up the mistake first.
Über diesen Sound
A 1.3-second clip of Prince John letting out a high, panicked scream, with no words, just a sudden cartoon shriek. It lands as funny and over-the-top, the sound of a pompous villain completely losing his composure when things go wrong.