Place this clip right after the reveal shot in your edit, then follow it with a wrong answer game show cue for a bigger fail, or precede it with sudden suspense to build toward the mishap before the reaction hits.
Über diesen Sound
This is a one-second voice clip of a scientist exclaiming oh no, delivered as a sharp, panicked outburst. It gives a listener an instant hit of alarmed disbelief and comic dread, like the moment a test result turns bad or an experiment slips out of control before anything can be fixed.