Place it as the payoff at the end of a rambling clip, after the speaker has had enough time to annoy the viewer. Follow it with the Silence I Kill You sound for a second beat, or let the No No Wait Wait sound lead into it when the interruption needs a longer build.
Über diesen Sound
This is a 1.7-second spoken clip of Ice Cube delivering a firm, two-word shut-up in his deep, direct voice. It lands as blunt and dismissive rather than angry, which makes the interruption feel funny instead of hostile. The abruptness does the work: there is no buildup, just a quick verbal door slam that tells the other person the conversation is over.