Place it at the peak of a workout or chase scene, just before the subject gives up, then follow it with the Dun Dun sting to sell the collapse. For a bigger payoff, add the Lego Yoda death gag right after it, or open with the Metal Gear alert before the struggle begins.
Über diesen Sound
This three-second clip features Duffman from The Simpsons gasping that he cannot breathe, followed by a worried oh no. The strained, theatrical delivery makes it feel funny and frantic at once, like a mascot collapsing mid-performance. It comes from the long-running animated series, where his over-the-top beer spokesman persona turns a simple breathless moment into a memorable gag.