Place this clip right after the line you want questioned, before your reaction shot cuts in. Follow it with the What Da Dog Doin Sound Button for a second beat, or end the sequence with a Nope Meme Sound Button refusal.
Über diesen Sound
This is a four-second voice clip of a person asking 'what did you sayyy,' with the last word stretched out for emphasis. It gives a feeling of playful disbelief and mock confrontation, like hearing something so unexpected or cheeky that it needs repeating back louder before anyone can respond to it properly.