Place it just after the moment a plan falls apart, then follow it with No No Wait Wait before the scene moves on, or cut to Oh My God Wow when the next reveal changes what everyone expected.
Über diesen Sound
This short spoken clip delivers the question 'wait, what are we doing?' in a puzzled, slightly exasperated voice, as if someone has just realised the group has lost the thread. It lands as pure confusion, funny because the hesitation feels familiar, and it works best as a quick reaction rather than a line that needs any setup.