Place it early in a night sequence, before any creature appears on screen, then follow it with the prowler sound effect for movement nearby and a fail sound meme only if you cut to a comic reaction shot afterwards.
Über diesen Sound
This is a 25-second recording of a lone howl set against a quiet wilderness backdrop, with the call carrying the whole clip. It feels mournful and eerie, making a night scene feel tense and lonely in a way a short bark or growl never could.