Place it just before the snake is shown, then follow it with Dun Dun Dun to build the moment, and finish with Vine Boom when the scare lands for a sharp comic payoff.
Über diesen Sound
This is a short, close-up snake hiss lasting just under three seconds, with a dry, raspy burst of air that rises and falls like a real snake warning something away. It feels tense and a little unsettling, the kind of sound that makes listeners pull back before they have time to think.