Place it right after a failed attempt in a clip, then follow with the Mission Failed sting to close the joke, or use the Ba Dum Tss cue when the rejection is meant as a punchline.
Über diesen Sound
This is a short spoken clip of a firm voice saying You can't do that in just over a second, with the words delivered as a direct, no-nonsense refusal. It feels funny and slightly awkward, like catching someone mid-mistake, because the flat correction lands before they can finish what they were doing.