Place this clip at the exact reveal in your edit, right after a Bell Ding Sound Effect Button marks the item appearing, then follow it with the Dun Dun Dun Sound Button if the lemon turns out to be a bad prize for your character.
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This is a sub-second lemon sound effect, a brief non-musical clip built around a single sharp lemon cue. It lasts just under one second, so it hits fast and clears out immediately. The feeling is tart and a little startling, like a sour twist dropped into a scene, which makes it read as playful rather than scary or heavy.