Place it at the end of a build-up, just after the setup line, then follow it immediately with a shocked reaction and an embarrassed laugh to sell the prank in the edit.
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This is a single, exaggerated fart sound lasting about 1.3 seconds, with a deep, wet blast that sounds deliberately over the top rather than subtle. It lands as crude, silly gross-out humour, the kind that makes a room laugh or groan at the same time, and it works best as a sudden interruption rather than background noise.