Place it at the start of the boss segment, after an Enemy Spotted cue warns viewers that trouble is coming and before the action peaks. Follow a defeat with the FNAF Jumpscare Scream for the payoff, or open with the Inception Horn to set up the fight.
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This 15-second clip is a Bowser battle sound, a short boss-fight cue centred on the big Mario villain and the showdown that comes with him. It feels tense, dramatic and exciting, with the kind of rising energy that makes a final fight feel bigger, riskier and harder to walk away from.