Place it as the final beat after the question appears on screen, once the setup has made the answer obvious. Lead in with No No Wait Wait to build hesitation, then let this clip close the exchange, and follow it with Wrong Answer Game Show when the rejected idea deserves one last judgment.
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This is a short spoken clip built around the phrase 'definitely not', delivered in a firm, flat voice and lasting just under three seconds. It lands as a blunt, comic refusal, the kind that shuts down a suggestion without leaving room to argue, and it works because the delivery sounds completely certain.