Put it right after the failed attempt in the edit, before any wider reaction shot. Follow it with the Emotional Damage meme sound when the loss is the main joke, or place the Bruh sound effect first when a quiet, deadpan setup makes the question land harder.
Sobre este sonido
This just-over-a-second voice clip has Japanese creator Hikakin asking “Kuyashikunai no?”, meaning “Aren’t you frustrated?” or “Doesn’t that bother you?” His delivery is quick, pointed and mocking, giving the line a teasing sting that lands without any setup. It comes from him, one of Japan’s best-known online creators, and spread as a short reaction in Japanese meme edits.