Place it right after a character disappears from the scene, then follow it with Bruh for a deadpan reaction or Emotional Damage when the search turns into a full-blown fail. Keep it near the start of a short edit so the missing-person gag sets up everything that follows.
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This 11-second clip is Luigi calling out 'Mario? Mario?' in a rising, uncertain voice, repeating his brother's name as if searching an empty room. The delivery feels anxious and a little lost, turning a simple call into a funny moment of panic. It captures Luigi's worried side, making it easy to picture him looking around for Mario.