Put it at the very end of a reveal, after a slow build-up, so it closes the scene. Pair it with Sudden Suspense just before the reveal, then follow it with Nelson's Ha-Ha! for a second laugh that keeps the joke going.
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This short evil laugh lasts about three seconds, a deep, theatrical chuckle that rises into a full maniacal cackle, the kind a cartoon villain lets out after a plan clicks into place. It feels mischievous and triumphantly over-the-top, turning an ordinary win into a moment of playful menace, with just enough wickedness to land as a joke rather than a threat.