Place this cue right after the wrong guess is shown, then follow it with the Windows XP Error sound for a second layer of failure. In a quiz edit, keep it after the question beat and before the next contestant's turn begins.
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This is the brief wrong-letter cue from Pole Chudes, the sound played when a contestant guesses a letter that is not in the hidden word. At about a second and a half, it lands as an immediate no. The feeling is deflating and a little tense, but also funny, because everyone watching knows the turn is lost in that instant.