Pesterchum is the fictional instant-messaging program used by the main characters in Homestuck, the webcomic by Andrew Hussie that began in 2009, and its chat sounds became part of the series' online identity as fans shared Pesterlogs, animations and edits. The end-of-conversation tone, like a Discord notification in function, marks the moment a character stops pestering another, which is why Homestuck fans recognise it instantly. It has stayed in circulation on soundboards and in fan videos because it condenses that very specific in-joke into about a second of audio.
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The Pesterchum end-of-message chime from Homestuck, played when a chat conversation closes. Fans use it for chat endings and Homestuck meme edits.
This is the brief notification that plays in Pesterchum when a conversation ends, a short electronic chime with a retro instant-messenger feel that lands cleanly and then stops. It is only about a second long, so it works as a punctuation mark rather than a melody, giving a chat exchange a clear sense of closure. For Homestuck fans it is instantly familiar, recalling John Egbert and his friends trading messages across the comic, while for new listeners it still reads as a tidy, slightly nostalgic alert. Used at the end of a clip, it makes a joke, argument or reveal feel finished.