Place it right after the failed moment in your edit, then follow it with the Nope Meme Sound for a sharper dismissal, or open with the Danger Alarm Meme Sound when the mistake is building up before the reveal.
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This is a roughly four-second descending buzzer horn from the game show The Price Is Right, played when a contestant gives a wrong answer. Its blunt, drawn-out tone feels awkward and instantly funny, turning a small failure into a clear joke. Because the cue is so familiar, listeners recognise the disappointment at once without any words.