Place it early in a scene, before the reveal, then follow it with the Inception Horn Sound Effect and finish with the FNAF Jumpscare Scream when the threat finally appears.
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This six-second clip is a distant, low roar from a huge ocean predator, echoing as if it is calling from far across open water. It feels tense and unsettling, building dread because the threat is heard long before it can be seen. It comes from Subnautica, where the Reaper Leviathan hunts players in the open ocean.