Place it right after the spider appears in your edit, following a build-up with Dun Dun Dun and before the reaction shot. Follow it with Vine Boom to punctuate the scare, then cut to the person stepping back.
À propos de ce son
This clip features Strong Bad and a spider, saved under the odd title Christina Aguilera Spider 2000. The pairing of a cartoon character with a creepy-crawly subject makes it a silly joke, giving it a goofy and slightly gross feeling that works as a sudden interruption. It comes from Strong Bad's spider bit.