Put it at the very end of a fail sequence, after the mishap has played out. Build tension first with Sudden Suspense, then let this clip close the joke. For a game-show style miss, follow it with Wrong Answer Game Show to underline the mistake.
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This is Nelson Muntz letting out his two-beat 'Ha-ha!' in a high, pinched voice, lasting just under two seconds. It feels gleefully smug, the kind of laugh that rubs a mistake in someone's face and makes the room laugh with the mocker. It comes from The Simpsons, where Nelson, the school bully, uses it as his signature put-down.