Bowser first appeared as the main villain in Nintendo's Super Mario Bros. in 1985 and has remained Mario's spiky, fire-breathing rival ever since. D'oh became famous as Homer Simpson's frustrated catchphrase on The Simpsons, one of the most quoted reaction lines in television. This clip brings the two together as a short fan style mashup that circulated on soundboards and in meme edits. Its appeal is the clash between a powerful game boss and a very human, embarrassed reaction, which is why creators keep reusing it for fail moments.