The clip comes from Old MacDonald Duck, a Donald Duck cartoon released on September 12, 1941, where the buzz fits the farmyard slapstick around Donald. It later circulated as a Hollywood Edge library sound, catalogued as Fly Buzz, which is why the same short buzz appears in many cartoon-style edits and soundboards. Its appeal has lasted because the sound is instantly recognisable: a single insect buzz needs no explanation, and in under a second it can signal irritation, dirtiness or an unwanted visitor. That simplicity keeps it useful for quick visual gags.