Use it as the closing beat of a short abandonment gag, after the exit has already happened on screen. Follow it with the Sad Meow Song to keep the focus on the kittens, or cut to the Emotional Damage sound when the joke is aimed at the person who left.
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This is a short Russian meme clip titled 'Cat Abandoned the Kittens,' lasting about seven and a half seconds. Built around that blunt, unhappy image, it works as a small, sad punchline rather than a loud joke, turning an awkward exit into something that feels worse than funny. It gives listeners a mix of sympathy for the kittens and dark humour at someone walking away.