This sound is credited to the Donald Duck cartoon Donald Duck and the Gorilla, released on March 31, 1944. The short is a classic Walt Disney Donald Duck cartoon where Donald and his nephews face an escaped gorilla, built around chase gags and exaggerated cartoon effects. Short animal noises like this hiccup and bubble pop were typical of that era's hand timed sound design. Online, editors lifted the moment as a standalone effect, and it now circulates on soundboards as a short reaction for silly, deflated or surprised scenes.
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Cartoon lion hiccups ending with a bubble pop from the 1944 Donald Duck short Donald Duck and the Gorilla, used for silly hiccup gags and surprise reactions.
This clip captures a cartoon lion letting out a series of hiccups that end with a soft bubble pop. The hiccups are short, high and bouncy rather than roaring, which makes the big animal sound unexpectedly small and silly. Each hiccup lands with a little pause in between, building a tiny rhythm before the bubble breaks the pattern. Played over a serious or proud moment, it instantly undercuts the mood and makes the character seem embarrassed, surprised or unable to keep it together, which is why it works so well as a quick comic puncture.