Nun Massacre is an indie survival horror game by Puppet Combo, first released in 2018, in which players explore a boarding school while being hunted by a murderous nun. Its chase sequences became a staple of horror playthroughs on YouTube and Twitch, where streamers and editors clipped the music for reaction videos and highlight reels. The sound remains popular because it signals danger instantly, even without visuals, and works as shorthand for being pursued in memes, edits and short horror content.