No verified source, character or original video is listed for Pussing Lol Bois, so it is best understood as a user-shared soundboard clip rather than a line from a known show, film or song. Its appeal comes from its title and its brevity: the phrase is odd enough to stick in memory, while the clip is short enough to reuse without derailing a video. That combination has kept it circulating as a small in-joke, passed between edits, streams and chats where a fast, unexpected reaction matters more than a recognisable reference.