Brook is the skeleton musician of the Straw Hat Pirates in Eiichiro Oda's One Piece, and yohohoho is his signature laugh, fitting his gentlemanly yet eccentric personality. In the scene referenced by fans, he bursts out laughing at the reveal connecting Sanji and Duval, making the clip a favourite reaction pull from the series. Like other distinctive One Piece laughs, it became shorthand online for over the top amusement, shared in compilations, edits and soundboards long after the episode aired because it needs no context to read as laughter.