Place it as the single punctuation hit after the setup, then follow it with the Vine Boom Sound Button for a bigger reaction and the Dun Dun Dun Sound Button when the reveal turns serious.
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This is a brief sound effect clip lasting about one and a half seconds, built around a single moment rather than a longer scene. It lands quickly and leaves a small jolt of surprise, the kind of quick hit that works as a reaction rather than a soundtrack.