Use it at the end of a failed joke in an edit, as the final beat before the scene cuts away. Follow it with the sad violin for mock sympathy, or let Nelson's ha-ha answer it when the joke deserves ridicule instead.
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This half-second reaction clip packs its joke into a single brisk burst, built around the count of 43 characters and the exasperated emphasis that follows it. It lands with dry, knowing amusement, the kind of sound for a gag everyone recognises instantly and has heard one time too many. Short, blunt and deliberately over it, it works best as a quick punctuation mark rather than a line to linger on.